Glial Cell Line-Derived Neurotrophic Factor Promotes Survival and Proliferation of Dental Pulp Cells
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چکیده
This study investigated the effects of glial cell line-derived neurotrophic factor (GDNF) on dental pulp stromal cells (DPCs). Cultures of DPCs expressed GDNF as well as its receptors, GFR1 and RET. Addition of recombinant GDNF to cultures in serumcontaining medium did not significantly affect DPC growth; however, GNDF dosedependently increased viable cell number under serum-free culture conditions. Live/dead, lactate dehydrogenase (LDH) and caspase -3,-7 assays demonstrated that cell death occurred under serum-free conditions, and that GDNF significantly reduced the number of dead cells by inhibiting apoptotic cell death. GDNF also stimulated cell proliferation in serum-free conditions, as assessed by the BrdU incorporation assay. The
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